Clinical Trials

Biography

Biography: Chih-Chang Chu

Abstract

A mast cell stabilizer (Ketotifen, KF) doped new biodegradable nano-fibrous membrane fabricated from a new synthetic amino acid-based poly(ester amides) (AA-PEA) and absorbable polycaprolactone (PCl), and hybrid hydrogels from Arg-PEA/chitosan derivatives were evaluated for wound healing characteristics of a 2^nd degree burn as well as full-thickness excision injury of a porcine model at 7, 14, and 21 days post-injury. The characteristics of the wound treated with the new biomaterials were evaluated in terms of tissue inflammatory responses and scar tissue formation. Wound tissues harvested were histologically evaluated for collagen content, type, mast cell and macrophage. Macrophages in tissue sections were labelled with ionized calcium binding adaptor molecule 1 (IBA1) and DAB as the chromagen. The proportion of type I vs. type III collagen was determined from photomicrographs of Pico Sirius Red stained slides illuminated with circularly polarized light, the ratio of yellow/red pixels to green pixels was determined using Fiji. Assay data were analyzed using ANOVA and appropriate post hoc tests for potential differences in time and/or treatment effects on wound HP content. Treatment of burns with the mast cell stabilizer-doped nano-fibrous membrane resulted in a lower wound hydroxyproline content than undoped membrane- or Silvadene-treated wounds, suggesting that the membranes themselves did not invoke a significant inflammatory reaction. Both IBA-1 and positive pixel count data of macrophage confirm that the KF-doped nanofibrous membranes showed lower numbers of macrophage (i.e., inflammation) than both the non-doped and Silvadent-treated burn wounds. There were no statistically significant differences in HP content between excision-wounded untreated and wounded hydrogel-treated groups, suggesting that the Arg-PEA hybrid gels did not induce significant inflammation.