Mohammadreza Sattari
Tabriz University of Medical Sciences, Iran
Title: Determination of glyceryl trinitrate and its two main metabolites in human plasma using a new sensitive gas chromatography method
Biography
Biography: Mohammadreza Sattari
Abstract
The aim of the present study was to determine the concentrations of nitroglycerin(glyceryl trinitrate, GTN, CAS 55-63-0) and its two main stable metabolites;1,2-dinitroglycerin (1,2-glyceryl dinitrate, GDN, CAS 621-65-8) and 1,3-dinitroglycerin(1,3-GDN, CAS 623-87-0) in human plasma using a capillary gas chromatography method with an electron capture detection. Using the GC conditions, linear calibrations were obtained for 1,3-GDN from 0.14 to 3 ng/mL, for 1,2-GDN from 0.06 to 6 ng/mL, and for GTN from 0.01 to0.3 ng/mL in plasma samples by the following calibration curve equations:[y=0.1924x–0.0088 (r2=0.999)],[y=0.2273x+0.0164 (r2=0.995)],[y=17.434x–0.0751] for 1,3-GDN, 1,2-GDN, and GTN respectively. The calculated limits of quantification values for GTN, 1,2-GDN, and 1,3-GDN were 0.03 ng/mL, 0.2 ng/mL, and 0.15 ng/mL respectively. This method was verified with a bioequivalence study of an Iranian brand of oral sustained release nitroglycerin with an innovator formulation.